This study illustrates the role that wetting and dewetting play in modulating protein–ligand interactions. Columns for screening of selectivity, binding and elution conditions, as well as small scale purifications. High-throughput resin for intermediate and polishing protein purification, excellent choice for process-scale purification. To explore how these differences in dewetting affect protein–ligand interactions, we calculated the probability of each ligand binding to different types of residues on the protein surface and found that the probability of binding to a hydrophobic residue followed the same order as the dewetting behavior. HiTrap Capto Phenyl ImpRes is prepacked with BioProcess Capto Phenyl ImpRes resins for screening and small-scale protein purifications using hydrophobic interaction chromatography. We then explored how solvation was perturbed when the ligand was bound to the protein surface and found that the probability of the phenyl ring dewetting followed the order: Capto MMC > Prototype 4 > Nuvia cPrime. First, the solvation characteristics of each ligand were quantified via three metrics: average water density, fluctuations, and residence time. Res resins for screening and small-scale protein purifications using hydrophobic interaction chromatography. Our investigation focused on three chromatography ligands: Capto MMC, Nuvia cPrime, and Prototype 4, a structural variant of Nuvia cPrime. The ligand structures of Capto adhere, Capto Q, Capto Phenyl, HEA Hypercel, and PPA Hypercel are shown (Figures adapted from GE Healthcare literature, Pall Corporation literature and 34). Here, we performed molecular dynamics simulations to explore how the ligand structure and geometry affect ligand–water interactions and how these differences in solution affect the nature of protein–ligand interactions. Recent studies have shown that selectivity in multimodal chromatographic separations is a function of the ligand structure and geometry. size (median particle size of the cumulative volume distribution) View Price and Availability Properties shelf life Please be aware this product may be shipped 90 days before the expiration date. Multimodal chromatography is a powerful approach for purifying proteins that uses ligands containing multiple modes of interaction. Capto S ImpAct Cytiva 17-3717-02, pack of 100 ml View Price and Availability GE17371502 Capto Adhere ImpRes, 100 ML Cytiva 17371502, 40 m avg.
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